LOCUS       V005486                 6949 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005486
VERSION     V005486
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Hashimoto-Gotoh T, Franklin FC, Nordheim A, Timmis KN.
  TITLE     Specific-purpose plasmid cloning vectors. I. Low copy number,
            temperature-sensitive, mobilization-defective pSC101-derived
            containment vectors
  JOURNAL   Gene 16 (1-3), 227-235 (1981)
   PUBMED   6282694
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Hashimoto-Gotoh T.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2002) Tam Hashimoto-Gotoh, Kyoto Pref. Univ. of
            Med., RINDG, Dept. of Biochem. and Mol. Genet.;
            Kawaramachi-Hirokoji, Kamigyo-Ku, Kyoto, Kyoto 602-8566, Japan
            (E-mail:e54093@sakura.kudpc.kyoto-u.ac.jp, Tel:81-75-251-5798,
            Fax:81-75-251-5799)
REFERENCE   3  (bases 1 to 6949)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6949)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 16
            (1-3), 227-235 (1981)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-NOV-2002) Tam Hashimoto-Gotoh, Kyoto Pref. Univ. of Med., RINDG,
            Dept. of Biochem. and Mol. Genet."; volume: " Kawaramachi-Hirokoji,
            Kamigyo-Ku, Kyoto, Kyoto 602-8566, Japan
            (E-mail:e54093@sakura.kudpc.kyoto-u.ac.jp, Tel:81-75-251-5798, Fax";
            pages: "81-75-251-5799"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(217..319)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      817..1039
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1087..2034
                     /label="Rep101(Ts)"
                     /note="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
     CDS             complement(2959..3303)
                     /gene="mbeC"
                     /label="Mobilization protein MbeC"
                     /note="Mobilization protein MbeC from Escherichia coli.
                     Accession#: P13657"
     CDS             complement(3503..4360)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4361..4465)
                     /label="AmpR promoter"
     CDS             complement(5063..5875)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             complement(join(6509..6949,1..216))
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
ORIGIN
        1 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
       61 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
      121 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
      181 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
      241 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
      301 acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg gcttcccggt
      361 atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca caggtattta
      421 ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta tgatggtgtt
      481 tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg
      541 gggtggtgcg taacggcaaa agcaccgccg gacatcagcg cctgtagtgc catttacccc
      601 cattcactgc cagagccgtg agcgcagcga actgaatgtc acgaaaaaga cagcgactca
      661 ggtgcctgat ggtcggagac aaaaggaata ttcagcgatt tgcccgagct tgcgagggtg
      721 ctacttaagc ctttagggtt ttaaggtctg ttttgtagag gagcaaacag cgtttgcgac
      781 atccttttgt aatactgcgg aactgactaa agtagtgagt tatacacagg gctgggatct
      841 attcttttta tcttttttta ttctttcttt attctataaa ttataaccac ttgaatataa
      901 acaaaaaaaa cacacaaagg tctagcggaa tttacagagg gtctagcaga atttacaagt
      961 tttccagcaa aggtctagca gaatttacag atacccacaa ctcaaaggaa aaggactagt
     1021 aattatcatt gactagccca tctcaattgg tatagtgatt aaaatcacct agaccaattg
     1081 agatgtatgt ctgaattagt tgttttcaaa gcaaatgaac tagcgattag tcgctatgac
     1141 ttaacggagc atgaaaccaa gctaatttta tgctgtgtgg cactactcaa ccccacgatt
     1201 gaaaacccta caaggaaaga acggacggta tcgttcactt ataaccaata cgttcagatg
     1261 atgaacatca gtagggaaaa tgcttatggt gtattagcta aagcaaccag agagctgatg
     1321 acgagaactg tggaaatcag gaatcctttg gttaaaggct ttgagatttt ccagtggaca
     1381 aactatgcca agttctcaag cgaaaaatta gaattagttt ttagtgaaga gatattgcct
     1441 tatcttttcc agttaaaaaa attcataaaa tataatctgg aacatgttaa gtcttttgaa
     1501 aacaaatact ctatgaggat ttatgagtgg ttattaaaag aactaacaca aaagaaaact
     1561 cacaaggcaa atatagagat tagccttgat gaatttaagt tcatgttaat gcttgaaaat
     1621 aactaccatg agtttaaaag gcttaaccaa tgggttttga aaccaataag taaagattta
     1681 aacacttaca gcaatatgaa attggtggtt gataagcgag gccgcccgac tgatacgttg
     1741 attttccaag ttgaactaga tagacaaatg gatctcgtaa ccgaacttga gaacaaccag
     1801 ataaaaatga atggtgacaa aataccaaca accattacat cagattccta cctacgtaac
     1861 ggactaagaa aaacactaca cgatgcttta actgcaaaaa ttcagctcac cagttttgag
     1921 gcaaaatttt tgagtgacat gcaaagtaag catgatctca atggttcgtt ctcatggctc
     1981 acgcaaaaac aacgaaccac actagagaac atactggcta aatacggaag gatctgaggt
     2041 tcttatggct cttgtatcta tcagtgaagc atcaagacta acaaacaaaa gtagaacaac
     2101 tgttcaccgt tagatatcaa agggaaaact gtccatatgc acagatgaaa acggtgtaaa
     2161 aaagatagat acatcagagc ttttacgagt ttttggtgca tttaaagctg ttcaccatga
     2221 acagatcgac aatgtaacag atgaacagca tgtaacacct aatagaacag gtgaaaccag
     2281 taaaacaaag caactagaac atgaaattga acacctgaga caacttgtta cagctcaaca
     2341 gtcacacata gacagcctga aacaggcgat gctgcttatc gaatcaaagc tgccgacaac
     2401 acgggagcca gtgacgcctc ccgtggggaa aaaatcatgg caattctgga agaaaatagc
     2461 gcctgtttcg tttcaggcag gttatcaggg agtgtcagcg tcctgcggtt ctccggggcg
     2521 ttcgggtcat gcagcccgta atggtgattt aacagcgtct gccaagcatc aattctaggc
     2581 ctgtctgcgc ggtcgtagta cggctggagg cgttttccgg tctgtagctc catgttcgga
     2641 atgacaaaat tcagctcaag ccgtcccttg tcctggtgct ccacccacag gatgctgtac
     2701 tgattttttt cgagaccggg catcagtaca cgctcaaagc tcgccatcac tttttcacgt
     2761 cctcccggcg gcagctcctt ctccgcgaac gacagaacac cggacgtgta tttcttcgca
     2821 aatggcgtgg catcgatgag ttcccggact tcttccggat taccctgaag caccgttgcg
     2881 ccttcgcggt tacgctccct ccccagcagg taatcaaccg gaccactgcc accacctttt
     2941 cccctggcat gaaatttaac tatcatcccg cgccccctgt tccctgacag ccagacgcag
     3001 ccggcgcagc tcatccccga tggccatcag tgcggccacc acctgaaccc ggtcaccgga
     3061 agaccactgc ccgctgttca ccttacgggc tgtctgattc aggttatttc cgatggcggc
     3121 cagctgacgc agtaacggcg gtgccagtgt cggcagtttt ccggaacggg caaccggctc
     3181 ccccaggcag acccgccgca tccataccgc cagttgttta ccctcacagc gttcaagtaa
     3241 ccgggcatgt tcatcatcag taacccgtat tgtgagcatc ctctcgcgtt tcatcggtat
     3301 cattacccca tgaacagaaa tcccccttac acggaggcat cagtgactaa acggggtctg
     3361 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3421 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3481 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3541 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3601 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3661 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3721 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3781 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     3841 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3901 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3961 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4021 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     4081 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     4141 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     4201 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     4261 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     4321 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4381 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     4441 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     4501 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     4561 ttcaagaatt ttataaaccg tggagcgggc aatactgagc tgatgagcaa tttccgttgc
     4621 accagtgccc ttctgatgaa gcgtcagcac gacgttcctg tccacggtac gcctgcggcc
     4681 aaatttgatt cctttcagct ttgcttcctg tcggccctca ttcgtgcgct ctaggatcct
     4741 ctacgccgga cgcatcgtgg ccggcatcac cggcgctgag gtctgcctcg tgaagaaggt
     4801 gttgctgact cataccaggc ctgaatcgcc ccatcatcca gccagaaagt gagggagcca
     4861 cggttgatga gagctttgtt gtaggtggac cagttggtga ttttgaactt ttgctttgcc
     4921 acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc agcaaaagtt
     4981 cgatttattc aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca
     5041 accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     5101 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     5161 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     5221 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     5281 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc
     5341 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     5401 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
     5461 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     5521 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     5581 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     5641 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     5701 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     5761 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     5821 tgttggaatt taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcataacac
     5881 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
     5941 cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa
     6001 cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg
     6061 caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg
     6121 gctccctcac tttctggctg gatgatgggg cgattcaggc ctggtatgag tcagcaacac
     6181 cttcttcacg aggcagacct cagcgctaac cgtttttatc aggctctggg aggcagaata
     6241 aatgatcata tcgtcaatta ttacctccac ggggagagcc tgagcaaact ggcctcaggc
     6301 atttgagaag cacacggtca cactgcttcc ggtagtcaat aaaccggtaa accagcaata
     6361 gacataagcg gctatttaac gaccctgccc tgaaccgacg accgggtcga atttgctttc
     6421 gaatttctgc cattcatccg cttattatca cttattcagg cgtagcacca ggcgtttaag
     6481 ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg cagtactgtt
     6541 gtaattcatt aagcattctg ccgacatgga agccatcaca gacggcatga tgaacctgaa
     6601 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     6661 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     6721 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     6781 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     6841 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     6901 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaa
//
